immunofluorescence microscopy

An Improved Immunofluorescence Microscopy for Observing Microtubule Cytoskeleton in Plant Cells

一种改进的观察植物细胞微管的免疫荧光染色方法

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METHODS: The interactions of tubeimoside with microtubule in CNE-2Z cells were investigated by immunofluorescence microscopy, and the interactions of tubeimoside with tubulin in CNE-2Z cells were detected by Western blotting.

方法：采用细胞免疫荧光法观察皂苷处理的CNE2Z细胞微管的变化，蛋白免疫印迹法分析皂苷处理的CNE2Z细胞中聚合和未聚合微管蛋白含量的变化。

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Immunofluorescence laser confocal microscopy was use to observed these cells after crawled on the slide.

细胞爬片，免疫荧光激光共聚焦显微镜观察。

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AIM: To investigate the effects of fluid shear stress on cytoskeleton of osteoblasts, the authors examined the effects applying confocal laser scanning microscope and Immunofluorescence microscopy.

目的：研究流体剪切力对成骨细胞骨架的影响。

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The intracellular calcium concentration was measured by laser confocal microscopy. The expressed proteins in the cells were determined by immunofluorescence microscopy and Western blot.

用激光共聚焦显微镜检测细胞内Ca~（2+）的含量，用免疫荧光和western blot法检测细胞内JWA蛋白的分布和表达南京医科大学硕士学位论文水平。

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Then tagged with a FLAG Candida antarctica lipase B protein with three kinds of C-terminal N-anchored fusion protein and used immunofluorescence microscopy, that the newly constructed yeast display common carrier is successful.

再以带有FLAG标签的南极假丝酵母脂肪酶B蛋白的C末端同3种锚定蛋白N端融合，并利用免疫荧光显微镜检测，证明新构建的毕赤酵母展示通用载体是成功的。

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Methods Reverse transcript polymerase chain reaction ( RT PCR), immunofluorescence microscopy and immunohistochemistry methods were used to detect P120 catenin expression in the cells and tissues mentioned above.

方法采用RT-PCR、免疫荧光标记（激光共聚焦显微镜）和免疫组织化学分析，分别对正常肝细胞、肝癌细胞、正常肝组织和肝癌组织中P120ctn进行检测。

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The spindle morphological change was observed by immunofluorescence staining and confocal microscopy.

免疫荧光及激光共聚焦显微镜观察MKN45细胞微管改变，流式细胞仪检测MKN45细胞周期及凋亡的变化；

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The expression of HN protein was visualized by Western blot and Immunofluorescence microscopy.

用western blot、免疫荧光法检测HN蛋白的表达。

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CD41 specific monoclonal antibody staining was observed by immunofluorescence microscopy.

利用CD41单克隆抗体免疫荧光染色观察培养体系中的细胞情况。

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The intracellular transport capabilities of these peptides were tested with indirect immunofluorescence assay and confocal microscopy.

采用间接免疫荧光与激光共聚焦显微镜技术，分别对上述18肽和9肽的穿膜能力进行了动态观察。

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The expression of α 3 and/ or α 5 subunits of nicotinic acetylcholine receptor in rat pituitary: double immunofluorescence microscopy study

烟碱型乙酰胆碱受体α3和/或α5型在大鼠脑垂体中的表达：荧光双标免疫组织化学研究

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Methods After anoxia/ oxidative stress, immunofluorescence staining and confocal microscopy were used to study the distribution of integrin α 3 on the membrane of TECs under 2 experimental conditions, i.e., fixed or cultured living cells.

方法采用免疫荧光激光共聚焦观察乙醇固定及培养活细胞两种实验条件下TEC整合素α3分布的染色结果。

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Methods The human placental villus-derived trophoblastic cell line ( HPT-8) in vitro was identified and assayed by light microscope, scanning electron microscope, transmission electron microscope, immunohistochemistry and immunofluorescence laser confocal microscopy.

方法通过光镜、扫描电镜、透射电镜、免疫组化和免疫荧光激光共聚焦的方法对体外培养出的HPT-8进行系统鉴定评价。

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Cells expressed fluorescent signal were observed in the different distribution of hepatic lobule under the immunofluorescence microscopy, considered possibly related to cell transplantation pathways, but were all distributed in the liver lobule periportal area or lobular perivascular site of more collagen deposition. 5.

免疫荧光显微镜下观察表达荧光信号的细胞在肝小叶内的分布不同，考虑可能与细胞移植途径有关，但均分布在肝小叶汇管区或小叶血管周围胶原纤维沉积较多的部位。

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Indirect ELISA test shows that Coupling of antibodies without influence of antigenicity. Immunofluorescence and immunogold electron microscopy revealed that Conjugates have specific targeting against Porcine Contagious Pleuropneumonia actinomycetes.

间接ELISA检测表明：二者的偶联对抗体的抗原性没有影响；免疫荧光及免疫电镜显示偶联物具有猪传染性胸膜肺炎放线菌特异性靶向性。

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Using immunofluorescence and immunoelectron microscopy, we show that Ca-TSP was present in granules and in vesicular structures of the oyster hemocyte.

利用免疫荧光和免疫电镜检测我们发现Ca-TSP位于牡蛎血淋巴细胞的颗粒及囊泡状结构中。

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Immunofluorescence double-staining and confocal microscopy were used to examine the configuration of subepithelial immune complex 、 C5b-9 and WT1 around the glomerular basement membrane ( GBM).

运用免疫荧光双套色法，在激光共聚焦荧光显微镜下观察上皮侧免疫复合物沉积构象、C5b-9和WT1在肾小球基膜侧的分布，并对患者肾小球足细胞进行密度定量分析。

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